tailieunhanh - Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:" Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import | Retrovirology BioMed Central Research Open Access Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type I integrase for efficient reverse transcription and viral DNA nuclear import Zhujun Ao1 2 3 Keith R Fowke2 Éric A Cohen3 and Xiaojian Yao 1 2 3 Address laboratory of Molecular Human Retrovirology Faculty of Medicine University of Manitoba Winnipeg Manitoba R3E 0W3 Canada 2Department of Medical Microbiology Faculty of Medicine University of Manitoba Winnipeg Manitoba R3E 0W3 Canada and 3Laboratory of Human Retrovirology Institut de Recherches Cliniques de Montreal Département de microbiologie et immunologie Faculte de Médecine Université de Montreal Montreal Quebec H2W 1R7 Canada Email Zhujun Ao - ao@ Keith R Fowke - fowkekr@ Éric A Cohen - Xiaojian Yao - yao2@ Corresponding author Published 18 October 2005 Received 05 August 2005 Accepted 18 October 2005 Retrovirology 2005 2 62 doi l 742-4690-2-62 This article is available from http content 2 1 62 2005 Ao et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In addition to mediating the integration process HIV-1 integrase IN has also been implicated in different steps during viral life cycle including reverse transcription and viral DNA nuclear import. Although the karyophilic property of HIV-1 IN has been well demonstrated using a variety of experimental approaches the definition of domain s and or motif s within the protein that mediate viral DNA nuclear import and its mechanism are still disputed and controversial. In this study we performed mutagenic analyses to investigate the contribution of different regions in the .