tailieunhanh - Báo cáo y học: "Ku protein as a potential human T-cell leukemia virus type 1 (HTLV-1) Tax target in clastogenic chromosomal instability of mammalian cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài:Ku protein as a potential human T-cell leukemia virus type 1 (HTLV-1) Tax target in clastogenic chromosomal instability of mammalian cells | Retrovirology BioMed Central Research Open Access Ku protein as a potential human T-cell leukemia virus type 1 HTLV-1 Tax target in clastogenic chromosomal instability of mammalian cells Franca Majone 1 Roberto Luisetto1 Daniela Zamboni1 Yoichi Iwanaga2 and Kuan-Teh Jeang2 Address Department of Biology University of Padua Padua Italy and 2Laboratory of Molecular Microbiology NIAID NIH Bethesda Maryland 20892-0460 USA Email Franca Majone - majone@ Roberto Luisetto - Daniela Zamboni - Yoichi Iwanaga - yoichiiwanaga@ Kuan-Teh Jeang - kj7e@ Corresponding author Published 13 July 2005 Received 13 June 2005 Retrovirology 2005 2 45 doi 1742-4690-2-45 Accepted 13 July 2005 This article is available from http content 2 1 45 2005 Majone et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The HTLV-1 Tax oncoprotein rapidly induces cytogenetic damage which can be measured by a significant increase in the number of micronuclei MN in cells. Tax is thought to have both aneuploidogenic and clastogenic effects. To examine the cellular target for Tax which might mechanistically explain the clastogenic phenomenon we tested the ability of Tax to induce MN in rodents cells genetically defective for either the Ku80 protein or the catalytic subunit of DNA protein kinase DNAPKcs . We found that cells genetically mutated in Ku80 were refractory to Tax s induction of MN while cells knocked-out for DNAPKcs showed increased number of Tax-induced MN. Using a cytogenetic method termed FISHI Fluorescent In Situ Hybridization and Incorporation which measures the number of DNA-breaks in cells that contained unprotected 3 -OH ends .