tailieunhanh - Báo cáo y học: " HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain | Retrovirology BioMed Central Research Open Access HIV-I Rev oligomerization is not obligatory in the presence of an extra basic domain Clemens Furnes1 Thomas Arnesen1 Peter Askjaer2 3 Jorgen Kjems2 and Anne Marie Szilvay 1 Address Department of Molecular Biology University of Bergen N-5020 Bergen Norway 2Department of Molecular Biology University of Aarhus DK-8000 Aarhus C Denmark and 3EMBL Heidelberg Germany Email Clemens Furnes - Thomas Arnesen - Peter Askjaer - Jorgen Kjems - kjems@ Anne Marie Szilvay - Corresponding author Published 10 June 2005 Received 25 April 2005 Retrovirology 2005 2 39 doi 1742-4690-2-39 Accepted I0 June 2005 This article is available from http content 2 1 39 2005 Furnes et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The HIV-1 Rev regulatory protein binds as an oligomeric complex to viral RNA mediating nuclear export of incompletely spliced and non-spliced viral mRNAs encoding the viral structural proteins. However the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear. Results The activity of various fusion proteins based on the negative oligomerization-defect Rev mutant M4 was tested using Rev dependent reporter constructs. An artificial M4 mutant dimer and an M4 mutant containing an extra basic domain from the HTLV-I Rex protein exhibited nearly full activity when compared to wild type Rev. Conclusion Rev dimerization appears to be required to expose free basic domains whilst the Rev oligomeric complex remains bound to viral RNA via other basic domains. Background The cytoplasmic .

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