tailieunhanh - Báo cáo y học: " Conservation of functional domains and limited heterogeneity of HIV-1 reverse transcriptase gene following vertical transmission"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Conservation of functional domains and limited heterogeneity of HIV-1 reverse transcriptase gene following vertical transmission | Retrovirology BioMed Central Research Open Access Conservation of functional domains and limited heterogeneity of HIV-1 reverse transcriptase gene following vertical transmission Vasudha Sundaravaradan Tobias Hahn and Nafees Ahmad Address Department of Microbiology and Immunology College of Medicine The University of Arizona Health Sciences Center Tucson Arizona 85724 USA Email Vasudha Sundaravaradan - vasudha@ Tobias Hahn - tobias@ Nafees Ahmad - nafees@ Corresponding author Published 26 May 2005 Received 18 February 2005 Retrovirology 2005 2 36 doi 1742-4690-2-36 Accepted 26 May 2005 This article is available from http content 2 1 36 2005 Sundaravaradan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The reverse transcriptase RT enzyme of human immunodeficiency virus type 1 HIV-1 plays a crucial role in the life cycle of the virus by converting the single stranded RNA genome into double stranded DNA that integrates into the host chromosome. In addition RT is also responsible for the generation of mutations throughout the viral genome including in its own sequences and is thus responsible for the generation of quasi-species in HIV-1-infected individuals. We therefore characterized the molecular properties of RT including the conservation of functional motifs degree of genetic diversity and evolutionary dynamics from five mother-infant pairs following vertical transmission. Results The RT open reading frame was maintained with a frequency of in five motherinfant pairs sequences following vertical transmission. There was a low degree of viral heterogeneity and estimates of genetic diversity in mother-infant pairs .