tailieunhanh - Báo cáo y học: " Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production. | Retrovirology BioMed Central Research Open Access Dual effect of the SR proteins ASF SF2 SC35 and 9G8 on HIV-I RNA splicing and virion production Sandrine Jacquenet1 2 Didier Decimo2 Delphine Muriaux2 and Jean- Luc Darlix 2 Address 1Laboratoire de Médecine et Thérapeutique moléculaire INSERM CIC9501 15 rue du Bois de la Champelle 54500 Vandoeuvre-lès-Nancy France and 2LaboRetro Unité de Virologie Humaine INSERM 412 Ecole Normale Supérieure de Lyon IFR 128 46 allée d Italie 69364 Lyon cedex 07 France Email Sandrine Jacquenet- Didier Decimo - ddecimo@ Delphine Muriaux - Jean-Luc Darlix - jldarix@ Corresponding author Published 22 May 2005 Received 02 May 2005 Retrovirology 2005 2 33 doi 1742-4690-2-33 Accepted 22 May 2005 This article is available from http content 2 1 33 2005 Jacquenet et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract In HIV-1 infected cells transcription of the integrated provirus generates the single full length 9 kb viral RNA a major fraction of which is spliced to produce the single-spliced 4 kb RNAs and the multiple-spliced 2 kb RNAs. These spliced RNAs are the messengers for the Env glycoproteins and the viral regulatory factors. The cellular SR and hnRNP proteins were shown in vitro to control alternative splicing by binding cis-regulatory elements on the viral RNA. To better understand in vivo the role of the SR proteins on HIV-1 genomic RNA splicing and virion production we used a human cell line expressing high levels of complete HIV-1 and either one of the ASF SF2 SC35 and 9G8 SR proteins. Results show that over-expressing SR proteins caused a large reduction of .