tailieunhanh - Báo cáo y học: " Functional characterization of two newly identified Human Endogenous Retrovirus coding envelope genes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Functional characterization of two newly identified Human Endogenous Retrovirus coding envelope genes. | Retrovirology BioMed Central Short report Open Access Functional characterization of two newly identified Human Endogenous Retrovirus coding envelope genes Sandra Blaiset2 Nathalie de Parsevah1 and Thierry Heidmann 1 Address 1Unité des Rétrovirus Endogènes et Eléments Rétroĩdes des Eucaryotes Supérieurs UMR 8122 CNRS Institut Gustave Roussy 39 rue Camille Desmoulins 94805 Villejuif Cedex France and 2Unité de Biologie des Rétrovirus Département de Virologie Institut Pasteur 25 rue du Dr Roux 75724 Paris cedex 15 France Email Sandra Blaise - sblaise@ Nathalie de Parseval - parseval@ Thierry Heidmann - heidmann@ Corresponding author tEqual contributors Published 14 March 2005 Received 27 January 2005 Retrovirology 2005 2 19 doi l742-4690-2-l9 Accepted I4 March 2005 This article is available from http content 2 l l9 2005 Blaise et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract A recent in silico search for coding sequences of retroviral origin present in the human genome has unraveled two new envelope genes that add to the 16 genes previously identified. A systematic search among the latter for a fusogenic activity had led to the identification of two bona fide genes named syncytin-l and syncytin-2 most probably co-opted by primate genomes for a placental function related to the formation of the syncytiotrophoblast by cell-cell fusion. Here we show that one of the newly identified envelope gene named envP b is fusogenic in an ex vivo assay but that its expression - as quantified by real-time RT-PCR on a large panel of human tissues - is ubiquitous albeit with a rather low value in most tissues. Conversely the second envelope gene named envV discloses a .