tailieunhanh - Báo cáo y học: " Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels. | Retrovirology BioMed Central Short report Open Access Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes intracellular visualization at ultrastructural resolution levels Nathalie J Arheh1 Sylvie Souquere-Besset2 and Pierre Charneau 1 Address 1Groupe de Virologie Moléculaire et Vectorologie Institut Pasteur 25-28 rue du Dr. Roux 75724 Paris France and 2Institut André Lwoff CNRS-FRE2937 7 rue Guy Moquet-BP8 94800 Villejuif France Email Nathalie J Arhel - arhel@ Sylvie Souquere-Besse - souquere@ Pierre Charneau - charneau@ Corresponding author tEqual contributors Published 26 June 2006 Received 29 March 2006 Retrovirology 2006 3 38 doi 1742-4690-3-38 Accepted 26 June 2006 This article is available from http content 3 1 38 2006 Arhel et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract HIV-1 and other lentiviruses have the unique ability among retroviruses to efficiently replicate in non-dividing cells as a result of the active nuclear import of their DNA genome across an interphasic nuclear membrane. Previous work has shown that a three-stranded DNA structure synthesized during HIV-1 reverse transcription called the central DNA flap acts as a cisdeterminant of HIV-1 genome nuclear import. Concordantly DNA Flap re-insertion in lentiviral-derived gene therapy vectors stimulates gene transfer efficiencies and complements the level of nuclear import to wild-type levels quantitatively indistinguishable from wild-type virus in all cell types and tissues examined so far. In order to define the precise nature of the replicative defect of DNA flap mutant viruses we carried out in situ DNA hybridization experiments with electron microscopy to .