tailieunhanh - Báo cáo y học: "Antigen-presenting particle technology using inactivated surface-engineered viruses: induction of immune responses against infectious agents"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Antigen-presenting particle technology using inactivated surface-engineered viruses: induction of immune responses against infectious agents. | Retrovirology BioMed Central Open Access Antigen-presenting particle technology using inactivated surface-engineered viruses induction of immune responses against infectious agents Joseph D Mosca Yung-Nien Chang and Gregory Williams Address JDM Technologies Inc. Ellicott City MD 21042 USA Email Joseph D Mosca - jdmosca@ Yung-Nien Chang - zhuxi50@ Gregory Williams - gvw3886@ Corresponding author Published 15 May 2007 Received 25 August 2006 Retrovirology 2007 4 32 doi 1742-4690-4-32 Accepted 15 May 2007 This article is available from http content 4 1 32 2007 Mosca et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Developments in cell-based and gene-based therapies are emerging as highly promising areas to complement pharmaceuticals but present day approaches are too cumbersome and thereby limit their clinical usefulness. These shortcomings result in procedures that are too complex and too costly for large-scale applications. To overcome these shortcomings we described a protein delivery system that incorporates over-expressed proteins into viral particles that are non-infectious and stable at room temperature. The system relies on the biological process of viral egress to incorporate cellular surface proteins while exiting their host cells during lytic and non-lytic infections. Results We report here the use of non-infectious surface-engineered virion particles to modulate immunity against three infectious disease agents - human immunodeficiency virus type 1 HIV-1 herpes simplex virus HSV and Influenza. Surface-engineering of particles are accomplished by genetic modification of the host cell surface that produces the egress budding viral .