tailieunhanh - Báo cáo y học: "Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells. | Retrovirology BioMed Central Open Access Short report Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells but more efficiently in AZT-treated cells Laurent Houzet Zakia Morichaud and Marylène Mougel Address CPBS UMI CNRS 4 bd Henri IV CS 69033 34965 Montpellier France Email Laurent Houzet - Zakia Morichaud - Marylène Mougel - Corresponding author Published 2 May 2007 Retrovirology 2007 4 30 doi 1742-4690-4-30 Received 28 March 2007 Accepted 2 May 2007 This article is available from http content 4 1 30 2007 Houzet et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ We have shown previously that HIV actively and selectively packages the spliced HIV RNAs into progeny virions. In the present study by using a RT-QPCR and QPCR strategies we show that spliced viral RNAs are present in infectious particles and consequently participate along with the unspliced genomic RNA to some of the early steps of infection such as the reverse transcription step. This work provides the first quantitative data on reverse transcription of the fully spliced viral RNAs also called the early transcripts in target cells but also inside virions. The latter results were obtained by measuring the natural endogenous reverse transcription activity directly on intact HIV-1 particles. Our study reveals that spliced HIV RNAs are reverse transcribed as efficiently as the genomic RNA both in cells and virions. Interestingly we also show that reverse transcription of spliced RNAs is 56-fold .