tailieunhanh - Báo cáo y học: "Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites. | Retrovirology BioMed Central Research Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites Martin R Jakobsent1 Joost Haasnoott2 Jesper Wengel3 Ben Berkhout2 and Jorgen Kjems 1 Open Access Address Department of Molecular Biology University of Aarhus . Mollers Allé building 130 DK-8000 Ẳrhus C Denmark 2Department of Human Retrovirology Academic Medical Center University of Amsterdam Meibergdreef 15 1105 AZ Amsterdam The Netherlands and 3Department of Chemistry University of Southern Denmark Campusvej 55 DK-5230 Odense M Denmark Email Martin R Jakobsen - mrj@ Joost Haasnoot - Jesper Wengel - jwe@ Ben Berkhout - Jorgen Kjems - jk@ Corresponding author tEqual contributors Published 26 April 2007 Received 8 February 2007 Retrovirology 2007 4 29 doi 1742-4690-4-29 Accepted 26 April 2007 This article is available from http content 4 1 29 2007 Jakobsen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background A primary concern when targeting HIV-1 RNA by means of antisense related technologies is the accessibility of the targets. Using a library selection approach to define the most accessible sites for 20-mer oligonucleotides annealing within the highly structured 5 -UTR of the HIV-1 genome we have shown that there are at least four optimal targets available. Results The biological effect of antisense DNA and LNA oligonucleotides DNA- and LNAzymes targeted to the four most accessible sites was tested for their abilities to block reverse

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