tailieunhanh - Báo cáo y học: " Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome. | Retrovirology BioMed Central Open Access Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome Pavlina Konstantinova1 Olivier ter Brake1 Joost Haasnoot1 Peter de Haan2 and Ben Berkhout 1 Address laboratory of Experimental Virology Department of Medical Microbiology Center for Infection and Immunity Amsterdam CINIMA Academic Medical Center University of Amsterdam Meibergdreef 15 1105 AZ Amsterdam The Netherlands and 2Viruvation B. V. Wassenaarseweg 72 2333 AL Leiden The Netherlands Email Pavlina Konstantinova - Olivier ter Brake - Joost Haasnoot - Peter de Haan - Ben Berkhout - Corresponding author Published I March 2007 Received 6 December 2006 Accepted 1 March 2007 Retrovirology 2007 4 15 doi 1742-4690-4-15 This article is available from http content 4 1 15 2007 Konstantinova et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Human immunodeficiency virus type 1 HIV-1 can be inhibited by means of RNA silencing or interference RNAi using synthetic short interfering RNAs siRNAs or gene constructs encoding short hairpin RNAs shRNAs or long hairpin RNAs lhRNAs . The use of siRNA and shRNA as antiviral therapeutic is limited because of the emergence of viral escape mutants. This problem is theoretically prevented by intracellular expression of lhRNAs generating multiple siRNAs that target the virus simultaneously thus reducing the chance of viral escape. However gene constructs encoding lhRNA molecules face problems with .