tailieunhanh - Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation

Inclusion bodies are insoluble protein aggregates usually found in recombi-nant bacteria when they are forced to produce heterologous protein species. These particles are formed by polypeptides that cross-interact through sterospecific contacts and that are steadily deposited in either the cell’s cytoplasm or the periplasm. | IFEBS Journal MINIREVIEW Biological role of bacterial inclusion bodies a model for amyloid aggregation Elena Garcia-Frutos1-3 Raimon Sabate1 4 Natalia S. de Groot1 4 Antonio Villaverde1-3 and Salvador Ventura1 4 1 Institute for Biotechnology and Biomedicine Universitat Autonoma de Barcelona Spain 2 Department of Genetics and Microbiology Universitat Autonoma de Barcelona Spain 3 CIBER de Bioingeniería Biomateriales y Nanomedicina CIBER-BBN Barcelona Spain 4 Department of Biochemistry and Molecular Biology Universitat Autonoma de Barcelona Spain Keywords aggregation amyloid FTIR inclusion bodies protein folding protein quality recombinant proteins Correspondence A. Villaverde Institute for Biotechnology and Biomedicine Universitat Autonoma de Barcelona Bellaterra 08193 Barcelona Spain Fax 34 93 581 2011 Tel 34 93 581 2148 E-mail S. Ventura Institute for Biotechnology and Biomedicine Universitat Autonoma de Barcelona Bellaterra 08193 Barcelona Spain Fax 34 93 581 2011 Tel 34 93 586 8956 E-mail These authors contributed equally to this work Received 28 January 2011 revised 18 March 2011 accepted 15 April 2011 doi Inclusion bodies are insoluble protein aggregates usually found in recombinant bacteria when they are forced to produce heterologous protein species. These particles are formed by polypeptides that cross-interact through sterospecific contacts and that are steadily deposited in either the cell s cytoplasm or the periplasm. An important fraction of eukaryotic proteins form inclusion bodies in bacteria which has posed major problems in the development of the biotechnology industry. Over the last decade the fine dissection of the quality control system in bacteria and the recognition of the amyloid-like architecture of inclusion bodies have provided dramatic insights on the dynamic biology of these aggregates. We discuss here the relevant aspects in the interface between cell .