tailieunhanh - Báo cáo khoa học: Signal peptide peptidase and its homologs in Arabidopsis thaliana – plant tissue-specific expression and distinct subcellular localization

Signal peptide peptidase (SPP) is an aspartic proteinase that hydrolyses its substrate within the plane of the cellular membrane. In vertebrates, it plays crucial roles in life processes such as differentiation, embryogenesis, cell signaling and immunological response. We first found SPP in plants. An ortholog of human SPP (AtSPP), and its five AtSPP homologs (AtSPPL1– AtSPPL5), were searched for in theArabidopsisdatabase. | ễFEBS Journal Signal peptide peptidase and its homologs in Arabidopsis thaliana - plant tissue-specific expression and distinct subcellular localization 1 -1 1 1- 2 1 2 1-3 Tomoko Tamura Tomiko Asakura Tomohiro Uemura Takashi Ueda Kaede Terauchi Takumi Misaka1 and Keiko Abe1 1 Department of Applied BiologicalChemistry Graduate Schoolof Agriculturaland Life Sciences The University of Tokyo Japan 2 Department of BiologicalSciences Graduate Schoolof Science The University of Tokyo Japan 3 Department of Health Science Hiroshima Institute of Technology Japan Keywords aspartic proteinase endoplasmic reticulum endosomes intramembrane-cleaving protease signal peptide peptidase Correspondence K. Abe Department of Applied Biological Chemistry Graduate Schoolof Agricultural and Life Sciences The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan Fax 81 3 5841 8006 Tel 81 3 5841 5129 E-mail aka7308@ T. Asakura Department of Applied BiologicalChemistry Graduate Schoolof Agriculturaland Life Sciences The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan Tel Fax 81 3 5841 1879 E-mail asakura@ Database Sequences are in the EMBL GenBank DNA Data Bank of Japan under the following accession numbers AtSPP AY065169 AtSPPL1 AY058181 AtSPPL2 AY062807 AtSPPL3 AK229438 AtSPPL4 AK229822 AtSPPL5 AB330675 Received 15 September 2007 revised 24 October 2007 accepted 30 October 2007 doi Signal peptide peptidase SPP is an aspartic proteinase that hydrolyses its substrate within the plane of the cellular membrane. In vertebrates it plays crucial roles in life processes such as differentiation embryogenesis cell signaling and immunological response. We first found SPP in plants. An ortholog of human SPP AtSPP and its five AtSPP homologs AtSPPLl-AtSPPL5 were searched for in the Arabidopsis database. These clones were grouped into three different clusters AtSPP was grouped with human SPP HsSPP .