tailieunhanh - Báo cáo khoa học: Isolation and characterization of the main small heat shock proteins induced in tomato pericarp by thermal treatment

In recent years, heat treatment has been used to prevent the development of chilling injury in fruits and vegetables. The acquired tolerance to chilling seen in treated fruit is related to the accumulation of heat shock proteins (HSPs). | ỊFEBS Journal Isolation and characterization of the main small heat shock proteins induced in tomato pericarp by thermal treatment Gustavo A. Polenta1 Juan J. Calvete2 and Claudia B. Gonzalez1 3 1 Institute de Tecnologia de Alimentos Institute Nacionalde Tecnologia Agropecuaria Castelar Argentina 2 Instituto de Biomedicina de Valencia Spain 3 Consejo Nacionalde Investigaciones Cientificas y Tecnicas CONICET Argentina Keywords chilling injury electrophoresis heat treatments protein purification stress proteins Correspondence G. A. Polenta Instituto de Tecnologia de Alimentos CNIA INTA CC77 B1708WAB Moron Buenos Aires Argentina Fax Tel 54 11 4621 0446 E-mail gpolenta@ Received 8 August 2007 revised 22 October 2007 accepted 24 October 2007 doi In recent years heat treatment has been used to prevent the development of chilling injury in fruits and vegetables. The acquired tolerance to chilling seen in treated fruit is related to the accumulation of heat shock proteins HSPs . The positive effect of heat treatment has generally been verified for only a narrow range of treatment intensities and more reliable methods of determining optimal conditions are therefore needed. In this regard quantitation of HSPs would seem to be an interesting tool for monitoring purposes. As a step toward the development of analytical methodology the objective of this study was the isolation and characterization of relevant HSPs from plant tissues. Tomato fruits were exposed to a temperature of 38 C for 0 3 20 and 27 h and protein extracts from pericarp were analysed using SDS PAGE. Analysis revealed the appearance of an intense 21 kDa protein band in treated samples. IEF of this band showed the presence of four major proteins HSPC1 HSPC2 HSPC3 and HSPC4 with similar pl values. A monospecific polyclonal antiserum was raised in rabbits against purified HSPC1 protein which cross-reacted with other small heat shock proteins. The major proteins were