tailieunhanh - Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress

Groups of oysters (Crassostrea gigas) were exposed to 25 C for 24 days (controls to 13 C) to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 C above the summer seawater temperature encountered in western Brittany, France where the animals were collected. | ễFEBS Journal Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress Anne-Leila Meistertzheim1 Arnaud Tanguy2 Dario Moraga1 and Marie-Therese Thebault1 1 Laboratoire des Sciences de 1 Environnement Marin Institut Universitaire European de la Mer Universite de Bretagne occidentale Plouzane France 2 Laboratoire Adaptation et Diversite en Milieu Marin Station Biologique Roscoff France Keywords climate Crassostrea gigas gene expression heat stress prolonged thermalstress Correspondence M. T. Thebault Laboratoire des Sciences de l Environnement Marin UMR-CNRS 6539 Institut Universitaire Europeen de la Mer Universite de Bretagne Occidentale Place Nicolas Copernic 29280 Plouzane France Fax 33 2 98 49 86 45 Tel 33 2 98 49 86 12 E-mail Received 5 April2007 revised 17 October 2007 accepted 19 October 2007 doi Groups of oysters Crassostrea gigas were exposed to 25 C for 24 days controls to 13 C to explore the biochemical and molecular pathways affected by prolonged thermal stress. This temperature is 4 C above the summer seawater temperature encountered in western Brittany France where the animals were collected. Suppression subtractive hybridization was used to identify specific up- and downregulated genes in gill and mantle tissues after 7-10 and 24 days of exposure. The resulting libraries contain 858 different sequences that potentially represent highly expressed genes in thermally stressed oysters. Expression of 17 genes identified in these libraries was studied using real-time PCR in gills and mantle at different time points over the course of the thermal stress. Differential gene expression levels were much higher in gills than in the mantle showing that gills are more sensitive to thermal stress. Expression of most transcripts mainly heat shock proteins and genes involved in cellular homeostasis showed a high and rapid increase at

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