tailieunhanh - Báo cáo khoa học: Phylogenetic analysis of the chromate ion transporter (CHR) superfamily

ChrA is a membrane protein that confers resistance to the toxic ion chro-mate through the energy-dependent chromate efflux from the cytoplasm. In the protein databases, ChrA is a member of the chromate ion transporter (CHR) superfamily, composed of at least several dozens of members, dis-tributed in the three domains of life. | ỊFEBS Journal Phylogenetic analysis of the chromate ion transporter CHR superfamily Cesar Diaz-Perez1 Carlos Cervantes1 Jesus Campos-Garcia1 Adriana Julian-Sanchez2 and Hector Riveros-Rosas2 1 Institute de Investigaciones Quimico-Biologicas Universidad Michoacana Morelia Michoacan Mexico 2 Departamento de Bioquimica Facultad de Medicina Universidad NacionalAutonoma de Mexico Mexico Keywords chromate resistance chromate transport Cupriavidus metallidurans phylogenetic analysis Pseudomonas aeruginosa Correspondence H. Riveros-Rosas Departamento de Bioquimica Facultad de Medicina Universidad NacionalAutonoma de Mexico Apdo. Postal70-159 Delegacion Coyoacan 04510 Mexico . Mexico Fax 52 55 5616 2419 Tel 52 55 5622 0829 E-mail hriveros@ Received 18 June 2007 revised 26 September 2007 accepted 12 October 2007 doi ChrA is a membrane protein that confers resistance to the toxic ion chromate through the energy-dependent chromate efflux from the cytoplasm. In the protein databases ChrA is a member of the chromate ion transporter CHR superfamily composed of at least several dozens of members distributed in the three domains of life. The aim of this work was to perform a phylogenetic analysis of the CHR superfamily. An exhaustive search for ChrA homologous proteins was carried out at the National Center for Biotechnology Information database. One hundred and thirty-five sequences were identified as members of the CHR superfamily 77 long-chain sequences or bidomains LCHR and 58 short-chain sequences or monodomains SCHR organized mainly as tandem pairs of genes whose resultant proteins probably possess oppositely oriented membrane topology. LCHR sequences were split into amino and carboxyl domains and the resultant domains were aligned with the SCHR proteins. A phylogenetic tree was reconstructed using four different methods obtaining similar results. The domains were grouped into three clusters the SCHR proteins cluster the .

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