tailieunhanh - Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae

cDNAs of the ecdysone receptor and the retinoid X receptor were cloned from the Japanese scorpion Liocheles australasiae, and the amino acid sequences were deduced. The full-length cDNA sequences of theL. austra-lasiae ecdysone receptor and the L. australasiaeretinoid X receptor were 2881 and 1977 bp in length, respectively, and the open reading frames encoded proteins of 560 and 414 amino acids. The amino acid sequence of the L. | ỊFEBS Journal Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae Yoshiaki Nakagawa Atsushi Sakai Fumie Magata Takehiko Ogura Masahiro Miyashita and Hisashi Miyagawa Division of Applied Life Sciences Graduate Schoolof Agriculture Kyoto University Kyoto Japan Keywords ecdysone receptor EcR Liocheles australasiae retinoid X receptor RXR scorpion ultraspiracle USP Correspondence Y. Nakagawa Division of Applied Life Sciences Graduate School of Agriculture Kyoto University Kyoto 606-8502 Japan Fax 81 75 7536123 Tel 81 75 7536117 E-mail naka@ Received 13 June 2007 revised 9 October 2007 accepted 11 October 2007 doi cDNAs of the ecdysone receptor and the retinoid X receptor were cloned from the Japanese scorpion Liocheles australasiae and the amino acid sequences were deduced. The full-length cDNA sequences of the L. austra-lasiae ecdysone receptor and the L. australasiae retinoid X receptor were 2881 and 1977 bp in length respectively and the open reading frames encoded proteins of 560 and 414 amino acids. The amino acid sequence of the L. australasiae ecdysone receptor was similar to that of the ecdysone receptor-A of the soft tick Ornithodoros moubata 68 and to that of the ecdysone receptor-A1 of the lone star tick Amblyomma americanum 66 but showed lower similarity to the ecdysone receptors of Orthoptera and Coleoptera 53-57 . The primary sequence of the ligand-binding region of the L. australasiae ecdysone receptor was highly homologous to that of ticks 85-86 . The amino acid sequence of the L. australasiae retinoid X receptor was also homologous to the amino acid sequence of ultraspiracles of ticks 63 and insects belonging to the orders Orthoptera and Coleop-tera 60-64 . The identity of both the L. australasiae ecdysone receptor and the L. australasiae retinoid X receptor to their lepidopteran and dip-teran orthologs was less than 50 . The cDNAs of both the