tailieunhanh - Báo cáo khoa học: Involvement of glutathione/glutathione S-transferase antioxidant system in butyrate-inhibited vascular smooth muscle cell proliferation

Vascular smooth muscle cell (VSMC) proliferation is an important etiologi-cal factor in vascular proliferative diseases such as primary atherosclerosis, hypertension, arterial and in-stent restenosis, and transplant vasculopathy. Our studies established that butyrate, a bacterial fermentation product of dietary fiber and a chromatin modulator, is a potent inhibitor of VSMC proliferation. | ễFEBS Journal Involvement of glutathione glutathione S-transferase antioxidant system in butyrate-inhibited vascular smooth muscle cell proliferation Kasturi Ranganna1 Omana P. Mathew1 Frank M. Yatsu2 Zivar Yousefipour1 Barbara E. Hayes1 and Shirlette G. Milton1 1 Department of PharmaceuticalSciences Texas Southern University Houston TX USA 2 Department of Neurology University of Texas Health Science Center at Houston TX USA Keywords butyrate glutathione glutathione S-transferase proliferation vascular smooth muscle cell Correspondence K. Ranganna Department of Pharmaceutical Sciences College of Pharmacy Texas Southern University 3100 Cleburne Street Houston TX 77004 USA Fax 1 713 313 1091 Tel 1 713 313 1886 E-mail Ranganna_kx@ Received 20 August 2007 revised 21 September 2007 accepted 25 September 2007 doi Vascular smooth muscle cell VSMC proliferation is an important etiological factor in vascular proliferative diseases such as primary atherosclerosis hypertension arterial and in-stent restenosis and transplant vasculopathy. Our studies established that butyrate a bacterial fermentation product of dietary fiber and a chromatin modulator is a potent inhibitor of VSMC proliferation. The cardiovascular health benefits of a high-fiber diet the principle source of butyrate in the body have been known for a long time however very little is known about the antiatherogenic potential of butyrate. Because oxidative stress plays an important role in the pathogenesis of atherosclerosis we examined involvement of the glutathione gluta-thione S-transferase GST antioxidant system in butyrate s inhibition of VSMC proliferation. Treatment of proliferating VSMCs with butyrate leads to the induction of several GSTs. Interestingly our study also demonstrated the nuclear localization of GST-P1 GST-7-7 which is considered to be a cytosolic protein this was demonstrated using immunostaining and was corroborated by western blotting. Also the .