tailieunhanh - Báo cáo khoa học: Natriuretic peptides affect Pseudomonas aeruginosa and specifically modify lipopolysaccharide biosynthesis

Natriuretic peptides of various forms are present in animals and plants, and display structural similarities to cyclic antibacterial peptides. Pretreat-ment ofPseudomonas aeruginosa PAO1 with brain natriuretic peptide (BNP) or C-type natriuretic peptide (CNP) increases bacterium-induced glial cell necrosis. In eukaryotes, natriuretic peptides act through receptors coupled to cyclases. | ễFEBS Journal Natriuretic peptides affect Pseudomonas aeruginosa and specifically modify lipopolysaccharide biosynthesis Wilfried Veron1 Olivier Lesouhaitier1 Xaviera Pennanec2 Karine Rehel2 Philippe Leroux3 Nicole Orange1 and Marc G. J. Feuilloley1 1 Laboratory of Cold Microbiology UPRES 2123 University of Rouen Evreux France 2 Laboratoire de Biotechnologie et Chimie Marines Universite de Bretagne-Sud . 92116 Lorient France 3 Research Department of Microenvironment and CellIntegrated Renewal UPRES 3829 University of Rouen France Keywords cyclases cytotoxicity natriuretic peptides sensor vfr Correspondence O. Lesouhaitier Laboratory of Cold Microbiology UPRES 2123 University of Rouen 55 rue Saint Germain 27000 Evreux France Fax 33 232 29 15 55 Tel 33 232 29 15 42 E-mail Received 18 July 2007 revised 28 August 2007 accepted 18 September 2007 doi Natriuretic peptides of various forms are present in animals and plants and display structural similarities to cyclic antibacterial peptides. Pretreatment of Pseudomonas aeruginosa PAO1 with brain natriuretic peptide BNP or C-type natriuretic peptide CNP increases bacterium-induced glial cell necrosis. In eukaryotes natriuretic peptides act through receptors coupled to cyclases. We observed that stable analogs of cAMP dibutyryl cAMP and cGMP 8-bromo-cGMP mimicked the effect of brain natriuretic peptide and CNP on bacteria. Further evidence for the involvement of bacterial cyclases in the regulation of P. aeruginosa PAO1 cytotoxicity by natriuretic peptides is provided by the observed doubling of intrabacte-rial cAMP concentration after exposure to CNP. Lipopolysaccharide LPS extracted from P. aeruginosa PAO1 treated with both dibutyryl cAMP and 8-bromo-cGMP induces higher levels of necrosis than LPS extracted from untreated bacteria. Capillary electrophoresis and MALDI-TOF MS analysis have shown that differences in LPS toxicity are due to specific .