tailieunhanh - Báo cáo hóa học: " A systems biology approach to analyse leaf carbohydrate metabolism in Arabidopsis thaliana"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A systems biology approach to analyse leaf carbohydrate metabolism in Arabidopsis thaliana | Henkel et al. EURASIP Journal on Bioinformatics and Systems Biology 2011 2011 2 http content 2011 1 2 s EURASIP Journal on Bioinformatics and Systems Biology a SpringerOpen Journal RESEARCH Open Access A systems biology approach to analyse leaf carbohydrate metabolism in Arabidopsis thaliana 1 t 2 t 2 3 11 Sebastian Henkel Thomas Nagele Imke Hormiller Thomas Sauter Oliver Sawodny Michael Ederer and Arnd G Heyer 2 Abstract Plant carbohydrate metabolism comprises numerous metabolite interconversions some of which form cycles of metabolite degradation and re-synthesis and are thus referred to as futile cycles. In this study we present a systems biology approach to analyse any possible regulatory principle that operates such futile cycles based on experimental data for sucrose Scr cycling in photosynthetically active leaves of the model plant Arabidopsis thaliana. Kinetic parameters of enzymatic steps in Scr cycling were identified by fitting model simulations to experimental data. A statistical analysis of the kinetic parameters and calculated flux rates allowed for estimation of the variability and supported the predictability of the model. A principal component analysis of the parameter results revealed the identifiability of the model parameters. We investigated the stability properties of Scr cycling and found that feedback inhibition of enzymes catalysing metabolite interconversions at different steps of the cycle have differential influence on stability. Applying this observation to futile cycling of Scr in leaf cells points to the enzyme hexokinase as an important regulator while the step of Scr degradation by invertases appears subordinate. Keywords Systems biology carbohydrate metabolism Arabidopsis thaliana kinetic modelling stability analysis sucrose cycling Introduction Plant metabolic pathways are highly complex comprising various branch points and crosslinks and thus kinetic modelling turns up as an adequate tool to investigate .