tailieunhanh - Báo cáo hóa học: " Investigations on antibody binding to a microcantilever coated with a BAM pesticide residue"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Investigations on antibody binding to a microcantilever coated with a BAM pesticide residue | Bache et al. Nanoscale Research Letters 2011 6 386 http content 6 1 386 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Investigations on antibody binding to a microcantilever coated with a BAM pesticide residue Michael Bache1 Rafael Taboryski1 Silvan Schmid1 Jens Aamand2 and Mogens Havsteen Jakobsen1 Abstract The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments using a cantilever-based detection system by Cantion A S. The stress induced by the binding of a pesticide residue BAM 2 6 dichlorobenzamide immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4 system from Cantion A S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4 system is a suited platform for a pesticide assay system. Introduction During the last 10 years an increasing number of water wells have been polluted by pesticides or its break down products. BAM is among the most frequent found pesticide residues in European groundwater. As pesticide analysis of drinking water is currently being done by laboratory analysis an in-line sensor will therefore be beneficial for water quality monitoring. Cantilever-based assays for pesticide detection has been reported 1 2 but few description of repeated measurements using cantilever-based detection systems are available. As a central principle of a possible cantilever-based competitive assay we have tested the binding of a BAM antibody to a cantilever surface passive coated with a BAM ovalbumine conjugate. In a working assay