tailieunhanh - DNA Methylation: Basic Mechanisms - Part 3

Hai báo cáo khác cho thấy, trái lại, RNA-methyl hóa DNA qua trung gian có thể xảy ra ở động vật có vú. Trong một nghiên cứu trên tế bào thận của con người, siRNA nhắm mục tiêu cho nhà sáng lập của phương tiện dẫn truyền lentiviral đã được tìm thấy sự im lặng gen EF1A nội sinh, im lặng là liên quan với methyl hóa DNA (Morris et al 2004). | DNA Methyltransferases Facts Clues Mysteries 61 suggest that dsRNA expsession while niu ucmiJi irt-transcriptional silencing by RNAi does not induce sequence-specific methylation of the cognate DNA sequence Svoboda et al. 2004 . Limitations to this study were that the system used was aHifineil io a spaaific cell type and that RdDiPtargelinxpvas analyzed in a single intionaaic endogenous reports supgesi cn dip contrary thalRNA-mediaied DNAmethylaIion pyn oocurin n ammi li. io one studymn humep kifneu csUf siRNA taefetpd toa promoierby maans of lentivtpai tsansfuption wss founO tp ailencpthe audoocnomeEFlA gene. silencing being with DNA Mprtii el p4 . In anpfher e t rtúa NAt pr ted iotda E-cadferin uene lu humae breast euf l cpi l Rills ipuaeat in taansseiniieeel repressíon Kawcstudand Taira p- whieh exnreation at DlMT gann wsu suypreised by meaniefTiRNAs tnrgesmf the pptrespindinn n ei r n-t imlRNAsPove shpwn tharDNMTl cnd DNMTin butnpeXNMT2i Ki likePn necessdty apr siRNA-medlxtedtran-paintlynN h itdngpn expes sei o trom tmcE-pahhesin papmptee. Bisulfite sehpanpini rcvesk ei c silenaing T0hrs lalee ond rety nae-s m c CpG mefOylatien ỊKowasaOiotlf Taiaa 2OS4 . Thi MUM appente huso ty oscusm mgmmytu Yeu from the few repprte cva0able la date ie wpcM alrcadysaem thnt. inducteci pfpNA methylati nbt í iRNin in it turns oxIIo oppur in mammols in rHmiled rauga ns ii will is 11 0X3 10 fplerminewhich eitdxelons andtoexplam ml n ooiy spma ie or some fems aip ebseeptiple 10 wip xfce underlyingmpshxnieme. Key puesiionswin be How xres-NNAs ganh no genomicfNAl ipw da chey gam aTceTl Alsiiwot thu ogrpepíal ottenliod given the meeNamrmyf RdXM itkmt wA bathe hole pieyef bycpeomelin-modifyinpa ie -rpm ft iixo snzymce anditu sequenecof Hants leadine siRNA-d-replenDNn metUhlatioTi .