tailieunhanh - báo cáo hóa học: " Enhancement of b-xylosidase productivity in cellulase producing fungus Acremonium cellulolyticus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Enhancement of b-xylosidase productivity in cellulase producing fungus Acremonium cellulolyticus | Kanna et al. AMB Express 2011 1 15 http content 1 1 15 o AMB Express a SpringerOpen Journal ORIGINAL Open Access Enhancement of p-xylosidase productivity in cellulase producing fungus Acremonium cellulolyticus 1 1 1 T- . I- 1 Machi Kanna Shinichi Yano Hiroyuki Inoue Tatsuya Fujii and Shigeki Sawayama Abstract Enzymatic hydrolysis is one of the most important processes in bioethanol production from lignocellulosic biomass. Acremonium cellulolyticus is a filamentous fungus with high cellulase production but productivity of hemicellulase especially p-xylosidase is lower than other filamentous fungi. We identified Kb p-xylosidase gene in the A. cellulolyticus genome sequence information and it encoded 798 amino acids without introns. To enhance hemicellulase productivity in A. cellulolyticus we transformed this fungus with the identified p-xylosidase gene driven by the cellobiohydrolase I cbh1 promoter using the protoplast-polyethyleneglycol PEG method and obtained a transformant YKX1. Hydrolysis rate of xylooligosaccharides was more than 50-fold higher using culture supernatant from YKX1 than that from the parental strain Y-94. Total cellulase activity measured by filter paper assay in YKX1 was not affected by the cbhl promoter used for expression of p-xylosidase and induced by cellulose. Since YKX1 can produce larger amount of p-xylosidase without affecting cellulase productivity it is considered to be beneficial for practical monosaccharide recoveries from lignocellulosic biomass. Keywords Hemicellulase beta-xylosidase Acremonium cellulolyticus Transformation Cellulase Introduction Ethanol produced from lignocellulosic biomass is a second-generation biofuel which does not compete with food resources Sims et al. 2010 and is expected to be an alternative to gasoline that reduces dependence on fossil fuels. Bioethanol can be produced from lignocellulosic biomass via several processes pretreatment enzymatic hydrolysis and fermentation. In .

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