tailieunhanh - báo cáo hóa học: " Effect of substrate (ZnO) morphology on enzyme immobilization and its catalytic activity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effect of substrate (ZnO) morphology on enzyme immobilization and its catalytic activity | Zhang et al. Nanoscale Research Letters 2011 6 450 http content 6 1 450 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Effect of substrate ZnO morphology on enzyme immobilization and its catalytic activity Yan Zhang1 Haixia Wu1 Xuelei Huang2 Jingyan Zhang2 and Shouwu Guo1 Abstract In this study zinc oxide ZnO nanocrystals with different morphologies were synthesized and used as substrates for enzyme immobilization. The effects of morphology of ZnO nanocrystals on enzyme immobilization and their catalytic activities were investigated. The ZnO nanocrystals were prepared through a hydrothermal procedure using tetramethylammonium hydroxide as a mineralizing agent. The control on the morphology of ZnO nanocrystals was achieved by varying the ratio of CH3OH to H2O which were used as solvents in the hydrothermal reaction system. The surface of as-prepared ZnO nanoparticles was functionalized with amino groups using 3-aminopropyltriethoxysilane and tetraethyl orthosilicate and the amino groups on the surface were identified and calculated by FT-IR and the Kaiser assay. Horseradish peroxidase was immobilized on as-modified ZnO nanostructures with glutaraldehyde as a crosslinker. The results showed that three-dimensional nanomultipod is more appropriate for the immobilization of enzyme used further in catalytic reaction. Introduction The composition size and surface characteristics of substrates have been considered as the important factors that affect the physical chemical properties of the immobilized enzymes 1-3 . However for conventional bulk solid substrates such as glass slides 4 polymer monoliths 5 6 and silica beads 3 the control on their morphologies and functionalizing their surfaces are usually laborious. Nanoscaled materials such as metal 7 and metal oxide nanospheres 8 carbon nanotubes 9 10 graphene oxide nanosheets 11 have also been utilized as the substrates for enzyme immobilization. It has been .

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