tailieunhanh - Báo cáo y học: " Effects of the K65R and K65R/M184V reverse transcriptase mutations in subtype C HIV on enzyme function and drug resistance"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Effects of the K65R and K65R/M184V reverse transcriptase mutations in subtype C HIV on enzyme function and drug resistance. | Retrovirology BioMed Central Research Effects of the K65R and K65R M184V reverse transcriptase mutations in subtype C HIV on enzyme function and drug resistance Hong-Tao Xu1 Jorge L Martinez-Cajas1 Michel L Ntemgwa1 2 Dimitrios Coutsinos1 2 3 Fernando A Frankel1 2 Bluma G Brenner1 2 3 and Mark A Wainberg 1 2 3 Address 1McGill University AIDS Centre Lady Davis Institute Jewish General Hospital Montreal Quebec H3T1E2 Canada 2Department of Medicine McGill University Montreal Quebec H3A 2T5 Canada and 3Department of Microbiology and Immunology McGill University Montreal Quebec H3A 2T5 Canada Email Hong-Tao Xu - hongtaoxu_00@ Jorge L Martinez-Cajas - Michel L Ntemgwa - Dimitrios Coutsinos - Fernando A Frankel - Bluma G Brenner - Mark AWainberg - Corresponding author Open Access Published II February 2009 Received 24 October 2008 Accepted 11 February 2009 Retrovirology 2009 6 14 doi 1742-4690-6-14 p This article is available from http content 6 1 14 2009 Xu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background We investigated the effects of mutations K65R and K65R plus M184V on enzymatic function and mechanisms of drug resistance in subtype C reverse transcriptase RT . Methods Recombinant subtype C HIV-1 RTs containing K65R or K65R M184V were purified from Escherichia coli. Enzyme activities and tenofovir TFV incorporation efficiency by wild-type WT and mutant RTs of both subtypes were determined in cell-free assays. Efficiency of - ssDNA synthesis and initiation by subtype C RTs was measured using .