tailieunhanh - Báo cáo y học: " Rapid spread of mouse mammary tumor virus in cultured human breast cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Rapid spread of mouse mammary tumor virus in cultured human breast cells. | Retrovirology BioMed Central Research Open Access Rapid spread of mouse mammary tumor virus in cultured human breast cells Stanislav Indik F2 Walter H Gunzburg1 2 Pavel Kulich3 Brian Salmons4 and Francoise Rouault2 4 Address Research Institute for Virology and Biomedicine University of Veterinary Medicine Vienna Vienna A-1210 Austria 2Christian-Doppler Laboratory for Gene Therapeutic Vector Development Vienna A-1210 Austria Veterinary Research Institute Brno 62100 Czech Republic and 4Austrianova Biotechnology GmbH Vienna A-1210 Austria Email Stanislav Indik - Walter H Gunzburg - Pavel Kulich - kulich@ Brian Salmons - salmons@ Francoise Rouault - rouault@ Corresponding author Published II October 2007 Received 31 May 2007 Accepted 1 1 October 2007 Retrovirology 2007 4 73 doi 1742-4690-4-73 This article is available from http content 4 1 73 2007 Indik et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The role of mouse mammary tumor virus MMTV as a causative agent in human breast carcinogenesis has recently been the subject of renewed interest. The proposed model is based on the detection of MMTV sequences in human breast cancer but not in healthy breast tissue. One of the main drawbacks to this model however was that until now human cells had not been demonstrated to sustain productive MMTV infection. Results Here we show for the first time the rapid spread of mouse mammary tumor virus MMTV GR in cultured human mammary cells Hs578T ultimately leading to the infection of every cell in culture. The replication of the virus was monitored by quantitative PCR quantitative RT-PCR and