tailieunhanh - Báo cáo y học: " Identification of a novel resistance (E40F) and compensatory (K43E) substitution in HIV-1 reverse transcriptase"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of a novel resistance (E40F) and compensatory (K43E) substitution in HIV-1 reverse transcriptase. | Retrovirology BioMed Central Research Identification of a novel resistance E40F and compensatory K43E substitution in HIV-1 reverse transcriptase Marleen CDG Huigen1 Petronella M van Ham1 Loek de Graaf1 Ron M Kagan2 Charles AB Boucher1 and Monique Nijhuis 1 Open Access Address Department of Medical Microbiology University Medical Center Utrecht The Netherlands and 2Department of Infectious Diseases Quest Diagnostics Incorporated 33608 Ortega Hwy San Juan Capistrano CA 92690 USA Email Marleen CDG Huigen - Petronella M van Ham - Loek de Graaf - Ron M Kagan - Charles AB Boucher - Monique Nijhuis - Corresponding author Published 13 February 2008 Received 7 June 2007 Retrovirology 2008 5 20 doi 1742-4690-5-20 Accepted 13 February 2008 This article is available from http content 5 1 20 2008 Huigen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background HIV-1 nucleoside reverse transcriptase inhibitors NRTIs have been used in the clinic for over twenty years. Interestingly the complete resistance pattern to this class has not been fully elucidated. Novel mutations in RT appearing during treatment failure are still being identified. To unravel the role of two of these newly identified changes E40F and K43E we investigated their effect on viral drug susceptibility and replicative capacity. Results A large database Quest Diagnostics database was analysed to determine the associations of the E40F and K43E changes with known resistance mutations. Both .