tailieunhanh - Báo cáo y học: " Intracellular HIV-1 Gag localization is impaired by mutations in the nucleocapsid zinc fingers"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Intracellular HIV-1 Gag localization is impaired by mutations in the nucleocapsid zinc fingers. | Retrovirology BioMed Central Research Intracellular HIV-1 Gag localization is impaired by mutations in the nucleocapsid zinc fingers Boyan Grigorov Didier Décimot1 Fatima Smagulova2 Christine Péchoux1 Marylène Mougel2 Delphine Muriaux 1 and Jean-Luc Darlix1 Open Access Address 1LaboRetro Unité de virologie humaine INSERM U758 IFR128 ENS 46 allée d Italie 69 364 Lyon France and 2CPBS UMI CNRS 4 bd Henri IV 34000 Montpellier France Email Boyan Grigorov - boyan78@ Didier Décimo - Fatima Smagulova - Christine Péchoux - cpechoux@ Marylène Mougel - Delphine Muriaux - dmuriaux@ Jean-Luc Darlix - jldarlix@ Corresponding author tEqual contributors Published 3 August 2007 Received 4 May 2007 Retrovirology 2007 4 54 doi 1742-4690-4-54 Accepted 3 August 2007 This article is available from http content 4 1 54 2007 Grigorov et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background The HIV-1 nucleocapsid protein NC is formed of two CCHC zinc fingers flanked by highly basic regions. HIV-1 NC plays key roles in virus structure and replication via its nucleic acid binding and chaperoning properties. In fact NC controls proviral DNA synthesis by reverse transcriptase RT gRNA dimerization and packaging and virion assembly. Results We previously reported a role for the first NC zinc finger in virion structure and replication 1 . To investigate the role of both NC zinc fingers in intracellular Gag trafficking and in virion assembly we generated series of NC zinc fingers mutations. .