tailieunhanh - Báo cáo y học: "Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors. | Retrovirology BioMed Central Open Access Host proteins interacting with the Moloney murine leukemia virus integrase Multiple transcriptional regulators and chromatin binding factors Barbara Studamire1 3 and Stephen P Goff 1 2 Address Department of Biochemistry and Molecular Biophysics Columbia University College of Physicians and Surgeons Hammer Health Sciences Center Room 1310c New York 10032 USA 2Howard Hughes Medical Institute Columbia University College of Physicians and Surgeons Hammer Health Sciences Center Room 1310c New York 10032 USA and 3Brooklyn College of CUNY 2900 Bedford Avenue Brooklyn NY 11210 USA Email Barbara Studamire - bstudamire@ Stephen P Goff - spg1@ Corresponding author Published 13 June 2008 Received 20 July 2007 Retrovirology 2008 5 48 doi 1742-4690-5-48 Accepted I3 June 2008 This article is available from http content 5 1 48 2008 Studamire and Goff licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background A critical step for retroviral replication is the stable integration of the provirus into the genome of its host. The viral integrase protein is key in this essential step of the retroviral life cycle. Although the basic mechanism of integration by mammalian retroviruses has been well characterized the factors determining how viral integration events are targeted to particular regions of the genome or to regions of a particular DNA structure remain poorly defined. Significant questions remain regarding the influence of host proteins on the selection of target sites on the repair of integration intermediates and on the efficiency of integration. Results We describe the results of a yeast two-hybrid screen using Moloney murine .