tailieunhanh - Báo cáo y học: " Role of receptor polymorphism and glycosylation in syncytium induction and host range variation of ecotropic mouse gammaretroviruses"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Role of receptor polymorphism and glycosylation in syncytium induction and host range variation of ecotropic mouse gammaretroviruses. | Retrovirology BioMed Central Research Role of receptor polymorphism and glycosylation in syncytium induction and host range variation of ecotropic mouse gammaretroviruses Yuhe Yan1 Yong T Jung2 Tiyun Wu3 and Christine A Kozak 1 Open Access Address laboratory of Molecular Microbiology National Institute of Allergy and Infectious Diseases Bethesda MD 20892-0460 USA 2Department of Microbiology Dankook University Cheonan 330-714 Korea and laboratory of Molecular Genetics National Institute of Child Health and Development Bethesda MD 20892 UsA Email Yuhe Yan - yyan@ Yong T Jung - yjung@ Tiyun Wu - wutiyun@ Christine A Kozak - ckozak@ Corresponding author Published 10 January 2008 Received II September 2007 Accepted 10 January 2008 Retrovirology 2008 5 2 doi 1742-4690-5-2 p This article is available from http content 5 1 2 2008 Yan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background We previously identified unusual variants of Moloney and Friend ecotropic mouse gammaretroviruses that have altered host range and are cytopathic in cells of the wild mouse species Mus dunni. Cytopathicity was attributed to different amino acid substitutions at the same critical env residue involved in receptor interaction S82F in the Moloney variant Spl574 and S84A in the Friend mouse leukemia virus F-S MLV. Because M. dunni cells carry a variant CAT-1 cell surface virus receptor dCAT-1 we examined the role of this receptor variant in cytopathicity and host range. Results We expressed dCAT-1 or mCAT-1 of NIH 3T3 origin in cells that are not normally infectible with .