tailieunhanh - Báo cáo y học: "Design of a trans protease lentiviral packaging system that produces high titer virus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Design of a trans protease lentiviral packaging system that produces high titer virus. | Retrovirology BioMed Central Research Design of a trans protease lentiviral packaging system that produces high titer virus Karen A Westerman 1 Zhujun Ao2 Eric A Cohen2 and Philippe Leboulch3 Open Access Address 1Brigham and Women s Hospital Department of Anesthesia SR157 75 Francis Street Boston MA 02115 USA 2Institut de Recherches Cliniques de Montréal and Department of Microbiology and Immunology Université of Montréal Quebec Canada and 3Genetics Division Department of Medicine and Harvard Medical School Brigham and Women s Hospital Harvard New Research Building Boston MA 02115 USA Email Karen A Westerman - kwest@ Zhujun Ao - ao@ Éric A Cohen - Philippe Leboulch - pleboulch@ Corresponding author Published 28 December 2007 Received 20 August 2007 Accepted 28 December 2007 Retrovirology 2007 4 96 doi l 742-4690-4-96 This article is available from http content 4 1 96 2007 Westerman et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The structural and enzymatic proteins of the human immunodeficiency virus HIV are initially generated as two long polyproteins encoded from overlapping reading frames one producing the structural proteins Gag and the second producing both structural and enzymatic proteins Gag-Pol . The Gag to Gag-Pol ratio is critical for the proper assembly and maturation of viral particles. To minimize the risk of producing a replication competent lentivirus RCL we developed a super-split lentiviral packaging system in which Gag was separated from Pol with minimal loss of transducibility by supplying protease PR intrans independently of both Gag and Pol. Results In developing this .