tailieunhanh - Báo cáo y học: " Dimerisation of HIV-2 genomic RNA is linked to efficient RNA packaging, normal particle maturation and viral infectivity"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " Dimerisation of HIV-2 genomic RNA is linked to efficient RNA packaging, normal particle maturation and viral infectivity. | Retrovirology BioMed Central Research Open Access Dimerisation of HIV-2 genomic RNA is linked to efficient RNA packaging normal particle maturation and viral infectivity Anne L Hernault1 Jane S Greatorex1 R Anthony Crowther2 and Andrew ML Lever 1 Address Department of Medicine University of Cambridge Addenbrooke s Hospital Cambridge CB2 2QQ UK and 2MRC Laboratory of Molecular Biology Cambridge CB2 0QH UK Email Anne L Hernault - al418@ Jane S Greatorex - jg10018@ R Anthony Crowther - rac1@ Andrew ML Lever - amll1@ Corresponding author Published 13 December 2007 Received 2 December 2007 Accepted 13 December 2007 Retrovirology 2007 4 90 doi 186 1742-4690-4-90 This article is available from http content 4 1 90 2007 L Hernault et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Retroviruses selectively encapsidate two copies of their genomic RNA the Gag protein binding a specific RNA motif in the 5 UTR of the genome. In human immunodeficiency virus type 2 HIV-2 the principal packaging signal Psi is upstream of the major splice donor and hence is present on all the viral RNA species. Cotranslational capture of the full length genome ensures specificity. HIV-2 RNA dimerisation is thought to occur at the dimer initiation site DIS located in stem-loop 1 SL-1 downstream of the main packaging determinant. However the HIV-2 packaging signal also contains a palindromic sequence pal involved in dimerisation. In this study we analysed the role of the HIV-2 packaging signal in genomic RNA dimerisation in vivo and its implication in viral replication. Results Using a series of deletion and substitution mutants in SL-1 and