tailieunhanh - Báo cáo y học: "Nuclear Factor 90(NF90) targeted to TAR RNA inhibits transcriptional activation of HIV-1"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Nuclear Factor 90(NF90) targeted to TAR RNA inhibits transcriptional activation of HIV-1. | Retrovirology BioMed Central Research Open Access Nuclear Factor 90 NF90 targeted to TAR RNA inhibits transcriptional activation of HIV-1 Emmanuel T Agbottaht Christine Travissh James McArdle Sambhav Karki Georges C St Laurent III and Ajit Kumar Address Department of Biochemistry Molecular Biology School of Medicine The George Washington University Washington . USA Email Emmanuel T Agbottah - bcmeta@ Christine Traviss - ctraviss@ James McArdle - jmcardle@ Sambhav Karki - skarki@ Georges C St Laurent - gsl@ Ajit Kumar - akumar@ Corresponding author tEqual contributors Published 12 June 2007 Received 19 January 2007 Retrovirology 2007 4 41 doi 1742-4690-4-41 Accepted 12 June 2007 This article is available from http content 4 1 41 2007 Agbottah et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Examination of host cell-based inhibitors of HIV-1 transcription may be important for attenuating viral replication. We describe properties of a cellular double-stranded RNA binding protein with intrinsic affinity for HIV-1 TAR RNA that interferes with Tat TAR interaction and inhibits viral gene expression. Results Utilizing TAR affinity fractionation North-Western blotting and mobility-shift assays we show that the C-terminal variant of nuclear factor 90 NF90ctv with strong affinity for the TAR RNA competes with Tat TAR interaction in vitro. Analysis of the effect of NF90ctv-TAR RNA interaction in vivo showed significant inhibition of Tat-transactivation of HIV-1 LTR in cells expressing NF90ctv as well as changes in histone H3 lysine-4 and lysine-9 methylation of HIV chromatin that are consistent with the epigenetic changes in .