tailieunhanh - Retrovirology Research BioMed Central Open Access Selective killing of HIV-1-positive macrophages

Retrovirology Research BioMed Central Open Access Selective killing of HIV-1-positive macrophages and T cells by the Rev-dependent lentivirus carrying anthrolysin O from Bacillus anthracis Jessica Young1, Zhongwei Tang1,2, Quan Yu3, Dongyang Yu1 and Yuntao Wu*1 Address: 1Department of Molecular and Microbiology, George Mason University, Manassas, VA 20110, USA, 2College of Life Sciences, Shanxi Agricultural University, Taigu, Shanxi 030801, P. R. China and 3Advanced Vision Therapies, Inc., Gaithersburg, MD 20878, USA Email: Jessica Young - jyoung4@; Zhongwei Tang - skybiotect@; Quan Yu - ; Dongyang Yu - dyu2@; Yuntao Wu* - ywu8@ * Corresponding author Published: 25 April 2008 Retrovirology 2008, 5:36 doi: Received: 20 December 2007 Accepted: 25. | Retrovirology BioMed Central Research Selective killing of HIV-1-positive macrophages and T cells by the Rev-dependent lentivirus carrying anthrolysin O from Bacillus anthracis Jessica Young1 Zhongwei Tang1 2 Quan Yu3 Dongyang Yu1 and Yuntao Wu 1 Open Access Address Department of Molecular and Microbiology George Mason University Manassas VA 20110 USA 2College of Life Sciences Shanxi Agricultural University Taigu Shanxi 030801 P. R. China and 3Advanced Vision Therapies Inc. Gaithersburg MD 20878 USA Email Jessica Young - jyoung4@ Zhongwei Tang - skybiotect@ Quan Yu - Dongyang Yu - dyu2@ Yuntao Wu - ywu8@ Corresponding author Published 25 April 2008 Received 20 December 2007 Retrovirology 2008 5 36 doi 1742-4690-5-36 Accepted 25 April 2008 This article is available from http content 5 1 36 2008 Young et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The ability of Human Immunodeficiency Virus HIV to persist in the body has proven to be a long-standing challenge to virus eradication. Current antiretroviral therapy cannot selectively destroy infected cells it only halts active viral replication. With therapeutic cessation or interruption viral rebound occurs and invariably viral loads return to pre-treatment levels. The natural reservoirs harboring replication-competent HIV-1 include CD4 T cells and macrophages. In particular cells from the macrophage lineage resist HIV-1-mediated killing and support sustained viral production. To develop a complementary strategy to target persistently infected cells this proof-of-concept study explores an HIV-1 Rev-dependent lentiviral vector carrying a bacterial hemolysin anthrolysin O anlO

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