tailieunhanh - Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model. | Retrovirology BioMed Central Research Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model Takashi Ohashi Mika Nagai Hiroyuki Okada Ryo Takayanagi and Hisatoshi Shida Open Access Address Division of Molecular Virology Institute for Genetic Medicine Hokkaido University Sapporo 060-0815 Japan Email Takashi Ohashi - ohashi-t@ Mika Nagai - purefood@ Hiroyuki Okada - hiro1230@ Ryo Takayanagi - coffea-arabica@ Hisatoshi Shida - hshida@ Corresponding author Published 8 October 2008 Received 22 July 2008 Retrovirology 2008 5 90 doi l742-4690-5-90 Accepted 8 October 2008 This article is available from http content 5 l 90 2008 Ohashi et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human T cell leukemia virus type I HTLV-I causes adult T-cell leukemia ATL in infected individuals after a long incubation period. Immunological studies have suggested that insufficient host T cell response to HTLV-I is a potential risk factor for ATL. To understand the relationship between host T cell response and HTLV-I pathogenesis in a rat model system we have developed an activation and detection system of HTLV-I Tax-specific cytotoxic T lymphocytes CTLs by Epitope expressing Single-Chain Trimers SCTs of MHC Class I. Results We have established expression vectors which encode SCTs of rat MHC-I with Tax180-188 peptide. Human cell lines transfected with the established expression vectors were able to induce IFN-Y and TNF-a production by a Taxl80-l88-specific CTL line 4OI C8. We have further fused the C-terminus of SCTs to EGFP and .