tailieunhanh - Báo cáo y học: "Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors. | Retrovirology BioMed Central Research Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors Mark D Andrake Monica M Sauter Kim Boland Andrew D Goldstein Maryem Hussein and Anna Marie Skalka Open Access Address Institute for Cancer Research Fox Chase Cancer Center Philadelphia PA 19111 USA Email Mark D Andrake - Monica M Sauter - msauter@ Kim Boland - Andrew D Goldstein - AndrewGoldstein@ Maryem Hussein - Anna Marie Skalka - AM_Skalka@ Corresponding author Published 7 August 2008 Received 5 May 2008 Retrovirology 2008 5 73 doi 1742-4690-5-73 Accepted 7 August 2008 This article is available from http content 5 1 73 2008 Andrake et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Integration of retroviral DNA into the host cell genome is an obligatory step in the virus life cycle. In previous reports we identified a sequence amino acids 201-236 in the linker region between the catalytic core and C-terminal domains of the avian sarcoma virus ASV integrase protein that functions as a transferable nuclear localization signal NLS in mammalian cells. The sequence is distinct from all known NLSs but like many contains basic residues that are essential for activity. Results Our present studies with digitonin-permeabilized HeLa cells show that nuclear import mediated by the NLS of ASV integrase is an active saturable and ATP-dependent process. As expected for transport through nuclear pore complexes import is blocked by treatment of cells with wheat germ agglutinin. We also show that import of ASV integrase requires soluble cellular factors but does not