tailieunhanh - Báo cáo y học: " APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: " APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication. | BioMed Central Retrovirology Open Access Research APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication Lin Li1 4 Dong Liang1 Jing-yun Li4 and Richard Y Zhao 1 2 3 4 Address Department of Pathology University of Maryland 10 South Pine Street MSTF700A Baltimore MD 21201 USA 2Department of Microbiology-Immunology University of Maryland 10 South Pine Street MSTF700A Baltimore MD 21201 USA institute of Human Virology University of Maryland 10 South Pine Street MSTF700A Baltimore MD 21201 USA and 4AIDS Research Department Beijing Institute of Microbiology and Epidemiology Beijing 100071 PR China Email Lin Li - dearwood@ Dong Liang - dliang@ Jing-yun Li - lijy@ Richard Y Zhao - rzhao@ Corresponding author Published 4 August 2008 Received 13 June 2008 Retrovirology 2008 5 72 doi 1742-4690-5-72 Accepted 4 August 2008 This article is available from http content 5 1 72 2008 Li et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Although APOBEC3G protein is a potent and innate anti-HIV-1 cellular factor HIV-1 Vif counteracts the effect of APOBEC3G by promoting its degradation through proteasome-mediated proteolysis. Thus any means that could prevent APOBEC3G degradation could potentially enhance its anti-viral effect. The UBA2 domain has been identified as an intrinsic stabilization signal that protects protein from proteasomal degradation. In this pilot study we tested whether APOBEC3G when it is fused with UBA2 can resist Vif-mediated proteasomal degradation and further inhibit HIV-1 .