tailieunhanh - Báo cáo y học: "Viral complementation allows HIV-1 replication without integration"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: Viral complementation allows HIV-1 replication without integration. | BioMed Central Retrovirology Research Viral complementation allows HIV-1 replication without integration Huub C Gelderblom11 Dimitrios N Vatakist2 Sean A Burke1 Steven D Lawrie1 Gregory C Bristol2 and David N Levy 1 Open Access Address Department of Basic Sciences and Craniofacial Biology New York University College of Dentistry New York NY USA and 2Department of Medicine David Geffen School of Medicine University of California Los Angeles Los Angeles CA USA Email Huub C Gelderblom - Dimitrios N Vatakis - dvatakis@ Sean A Burke - sab541 @ Steven D Lawrie - sdlawrie@ Gregory C Bristol - gbristol@ David N Levy - dnlevy@ Corresponding author tEqual contributors Published 9 July 2008 Received 9 May 2008 Retrovirology 2008 5 60 doi 1742-4690-5-60 Accepted 9 July 2008 This article is available from http content 5 1 60 2008 Gelderblom et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA has been reported but viral replication does not proceed further in cells which contain only unintegrated DNA. Multiple infection of cells is common and cells that are productively infected with an integrated provirus frequently also contain unintegrated HIV-1 DNA. Here we examine the influence of an integrated provirus on unintegrated HIV-1 DNA uDNA . Results We employed reporter viruses and quantitative real time PCR to .