tailieunhanh - báo cáo khoa học: " Novel proteins associated with risk for coronary heart disease or stroke among postmenopausal women identified by in-depth plasma proteome profiling"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Novel proteins associated with risk for coronary heart disease or stroke among postmenopausal women identified by in-depth plasma proteome profiling | Prentice et al. Genome Medicine 2010 2 48 http content 2 7 48 Genome Medicine RESEARCH Open Access Novel proteins associated with risk for coronary heart disease or stroke among postmenopausal women identified by in-depth plasma proteome profiling 1 2 1 11 1 Ross L Prentice Sophie J Paczesny Aaron Aragaki Lynn M Amon Lin Chen Sharon J Pitteri 11 13 4 5 Martin McIntosh Pei Wang Tina Buson Busald Judith Hsia Rebecca D Jackson Jacques E Rossouw JoAnn E Manson6 Karen Johnson7 Charles Eaton8 Samir M Hanash 1 Abstract Background Coronary heart disease CHD and stroke were key outcomes in the Women s Health Initiative WHI randomized trials of postmenopausal estrogen and estrogen plus progestin therapy. We recently reported a large number of changes in blood protein concentrations in the first year following randomization in these trials using an in-depth quantitative proteomics approach. However even though many affected proteins are in pathways relevant to the observed clinical effects the relationships of these proteins to CHD and stroke risk among postmenopausal women remains substantially unknown. Methods The same in-depth proteomics platform was applied to plasma samples obtained at enrollment in the WHI Observational Study from 800 women who developed CHD and 800 women who developed stroke during cohort follow-up and from 1-1 matched controls. A plasma pooling strategy followed by extensive fractionation prior to mass spectrometry was used to identify proteins related to disease incidence and the overlap of these proteins with those affected by hormone therapy was examined. Replication studies using enzyme-linked-immunosorbent assay ELISA were carried out in the WHI hormone therapy trial cohorts. Results Case versus control concentration differences were suggested for 37 proteins nominal P for CHD with three proteins beta-2 microglobulin B2M alpha-1-acid glycoprotein 1 ORM1 and insulin-like growth factor binding protein acid labile subunit .