tailieunhanh - Báo cáo khoa học: " Early changes of CD4-positive lymphocytes and NK cells in patients with severe Gram-negative sepsis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Early changes of CD4-positive lymphocytes and NK cells in patients with severe Gram-negative sepsis. | Available online http content 10 6 R166 Research Early changes of CD4-positive lymphocytes and NK cells in patients with severe Gram-negative sepsis Evangelos J Giamarellos-Bourboulis Thomas Tsaganos Ekaterini Spyridaki Maria Mouktaroudi Diamantis Plachouras Ilia Vaki Vassiliki Karagianni Anastasia Antonopoulou Vassiliki Veloni and Helen Giamarellou Open Access 4th Department of Internal Medicine University of Athens Medical School 1 Rimini Str. Athens 124 62 Greece Corresponding author Evangelos J Giamarellos-Bourboulis giamarel@ Received 2 Sep 2006 Revisions requested 29 Sep 2006 Revisions received 21 Oct 2006 Accepted 27 Nov 2006 Published 27 Nov 2006 Critical Care 2006 10 R166 doi cc5111 This article is online at http content 10 6 R166 2006 Giamarellos-Bourboulis et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Our aim was to define early changes of lymphocytes and of NK cells in severe sepsis and to correlate them with serum levels of soluble triggering receptor expressed on myeloid cells-1 sTREM-1 . Methods Blood was sampled from 49 patients with proven highly suspected infection by Gram-negative pathogens within 12 hours of the advent of severe sepsis and was also sampled from six healthy volunteers. White blood cells were targeted with monoclonal antibodies and were analyzed by flow cytometry. The concentrations of sTREM-1 were estimated by ELISA. Results The presence of CD3 CD4 cells was significantly lower P and that of NK cells significantly higher among patients with sepsis compared with controls P . The proportions median standard error of ANNEXIN-V CD4 CD3-positive cells of ANNEXIN-V CD8 CD3-positive cells and of ANNEXIN-V