tailieunhanh - báo cáo khoa học: " Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair | Amado et al. Journal of NeuroEngineering and Rehabilitation 2010 7 7 http content 7 1 7 l dl JOURNAL OF NEUROENGINEERING NCR AND REHABILITATION RESEARCH Open Access Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair 3t 3 1 1 Sandra Amado Jorge M Rodrigues Ana L Luis Paulo AS Armada-da-Silva Márcia Vieira Andrea Gartner I Qimõcic1 Ạ r ii Ar 1 D olr icr t3 Rnm m4 D imr inHr t4 Arfi ir QD W roiSr i5 fOcii m24 Mana J Simões António P Veloso Michele Foinaio Stefania Raimondo AILUI SP Vaiejão Stefano Geuna Ana C Mauricio1 2 Abstract Peripheral nerves possess the capacity of self-regeneration after traumatic injury but the extent of regeneration is often poor and may benefit from exogenous factors that enhance growth. The use of cellular systems is a rational approach for delivering neurotrophic factors at the nerve lesion site and in the present study we investigated the effects of enwrapping the site of end-to-end rat sciatic nerve repair with an equine type III collagen membrane enriched or not with N1E-115 pre-differentiated neural cells. After neurotmesis the sciatic nerve was repaired by end-to-end suture End-to-End group end-to-end suture enwrapped with an equine collagen type III membrane End-to-EndMemb group and end-to-end suture enwrapped with an equine collagen type III membrane previously covered with neural cells pre-differentiated in vitro from N1E-115 cells End-to-EndMembCell group . Along the postoperative motor and sensory functional recovery was evaluated using extensor postural thrust EPT withdrawal reflex latency WRL and ankle kinematics. After 20 weeks animals were sacrificed and the repaired sciatic nerves were processed for histological and stereological analysis. Results showed that enwrapment of the rapair site with a collagen membrane with or without neural cell enrichment did not lead to any significant improvement in most of