tailieunhanh - Báo cáo khoa học: " Systemic hypothermia increases PAI-1 expression and accelerates microvascular thrombus formation in endotoxemic mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Systemic hypothermia increases PAI-1 expression and accelerates microvascular thrombus formation in endotoxemic mice. | Available online http content 10 5 R148 Research Systemic hypothermia increases PAI-1 expression and accelerates microvascular thrombus formation in endotoxemic mice Nicole Lindenblatt1 2 Michael D Menger3 Ernst Klar2 and Brigitte Vollmar1 Department of Experimental Surgery University of Rostock Schillingallee Rostock 18055 Germany department of General Surgery University of Rostock Schillingallee Rostock 18055 Germany institute for Clinical and Experimental Surgery University of Saarland Kirrberger StraBe Homburg-Saar 66424 Germany Corresponding author Brigitte Vollmar Received 18 Jul 2006 Revisions requested 26 Jul 2006 Revisions received 15 Aug 2006 Accepted 24 Oct 2006 Published 24 Oct 2006 Critical Care 2006 10 R148 doi cc5074 This article is online at http content 10 5 R148 2006 Lindenblatt et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction Hypothermia during sepsis significantly impairs patient outcome in clinical practice. Severe sepsis is closely linked to activation of the coagulation system resulting in microthrombosis and subsequent organ failure. Herein we studied whether systemic hypothermia accelerates microvascular thrombus formation during lipopolysacharide LPS -induced endotoxemia in vivo and characterized the low temperature-induced endothelial and platelet dysfunctions. Methods Ferric-chloride induced microvascular thrombus formation was analyzed in cremaster muscles of hypothermic endotoxemic mice. Flow cytometry ELISA and immunohistochemistry were used to evaluate the effect of hypothermia on endothelial and platelet function. Results Control animals at 37 C revealed complete occlusion of .