tailieunhanh - báo cáo khoa học: "Association analysis of frost tolerance in rye using candidate genes and phenotypic data from controlled, semi-controlled, and field phenotyping platforms"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Association analysis of frost tolerance in rye using candidate genes and phenotypic data from controlled, semi-controlled, and field phenotyping platforms | Li et al. BMC Plant Biology 2011 11 146 http 1471-2229 11 146 BMC Plant Biology RESEARCH ARTICLE Open Access Association analysis of frost tolerance in rye using candidate genes and phenotypic data from controlled semi-controlled and field phenotyping platforms 1t 2t 13 3 1 4 longle LI Andreas Duck Gilt ndseneyei Viktor Korzun reel Wilde Clins Caromi Jinun DUnnd r Ankerst and Eva Dduer 1 Abstract Background Frost is an important abiotic stress that limits cereal production in the temperate zone. As the most frost tolerant small grain cereal rye Secale cereale L. is an ideal cereal model for investigating the genetic basis of frost tolerance FT a complex trait with polygenic inheritance. Using 201 genotypes from five Eastern and Middle European winter rye populations this study reports a multi-platform candidate gene-based association analysis in rye using 161 single nucleotide polymorphisms SNPs and nine insertion-deletion Indel polymorphisms previously identified from twelve candidate genes with a putative role in the frost responsive network. Results Phenotypic data analyses of FT in three different phenotyping platforms controlled semi-controlled and field revealed significant genetic variations in the plant material under study. Statistically significant P associations between FT and SNPs haplotypes of candidate genes were identified. Two SNPs in ScCbf15 and one in ScCbf12 all leading to amino acid exchanges were significantly associated with FT over all three phenotyping platforms. Distribution of SNP effect sizes expressed as percentage of the genetic variance explained by individual SNPs was highly skewed towards zero with a few SNPs obtaining large effects. Two-way epistasis was found between 14 pairs of candidate genes. Relatively low to medium empirical correlations of SNP-FT associations were observed across the three platforms underlining the need for multi-level experimentation for dissecting complex associations between .