tailieunhanh - báo cáo khoa học: "Transcript profiling reveals expression differences in wild-type and glabrous soybean lines"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcript profiling reveals expression differences in wild-type and glabrous soybean lines | BMC Plant Biology Clark standard Clark glabrous Wild type Hairless soybean mutant Transcript profiling reveals expression differences in wild-type and glabrous soybean lines Hunt et al. Hunt et al. BMC Plant Biology 2011 11 145 http 1471-2229 11 145 26 October 2011 BioMed Central Hunt et al. BMC Plant Biology 2011 11 145 http 1471-2229 11 145 BMC Plant Biology RESEARCH ARTICLE Open Access Transcript profiling reveals expression differences in wild-type and glabrous soybean lines Matt Hunt1 3t Navneet KaurH Martina Stromvik2 and Lila Vodkin1 Abstract Background Trichome hairs affect diverse agronomic characters such as seed weight and yield prevent insect damage and reduce loss of water but their molecular control has not been extensively studied in soybean. Several detailed models for trichome development have been proposed for Arabidopsis thaliana but their applicability to important crops such as cotton and soybean is not fully known. Results Two high throughput transcript sequencing methods Digital Gene Expression DGE Tag Profiling and RNA-Seq were used to compare the transcriptional profiles in wild-type cv. Clark standard CS and a mutant cv. Clark glabrous . trichomeless or hairless CG soybean isoline that carries the dominant P1 allele. DGE data and RNA-Seq data were mapped to the cDNAs Glyma models predicted from the reference soybean genome Williams 82. Extending the model length by 250 bp at both ends resulted in significantly more matches of authentic DGE tags indicating that many of the predicted gene models are prematurely truncated at the 5 and 3 UTRs. The genome-wide comparative study of the transcript profiles of the wild-type versus mutant line revealed a number of differentially expressed genes. One highly-expressed gene Glyma04g35130 in wild-type soybean was of interest as it has high homology to the cotton gene GhRDLl gene that has been identified as being involved in cotton fiber initiation and is a