tailieunhanh - báo cáo khoa học: "Localization of QTLs for in vitro plant regeneration in tomato"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Localization of QTLs for in vitro plant regeneration in tomato | BMC Plant Biology Localization of QTLs for in vitro plant regeneration in tomato Trujillo-Moya et al. Trujillo-Moya et al. BMC Plant Biology 2011 11 140 http 1471-2229 11 140 20 October 2011 BioMed Central Trujillo-Moya et al. BMC Plant Biology 2011 11 140 http 1471-2229 11 140 BMC Plant Biology RESEARCH ARTICLE Open Access Localization of QTLs for in vitro plant regeneration in tomato Carlos Trujillo-Moya1 Carmina Gisbert 1 Santiago Vilanova and Fernando Nuez Abstract Background Low regeneration ability limits biotechnological breeding approaches. The influence of genotype in the regeneration response is high in both tomato and other important crops. Despite the various studies that have been carried out on regeneration genetics little is known about the key genes involved in this process. The aim of this study was to localize the genetic factors affecting regeneration in tomato. Results We developed two mapping populations F2 and BC1 derived from a previously selected tomato cultivar cv. Anl27 with low regeneration ability and a high regeneration accession of the wild species Solanum pennellii PE-47 . The phenotypic assay indicated dominance for bud induction and additive effects for both the percentage of explants with shoots and the number of regenerated shoots per explant. Two linkage maps were developed and six QTLs were identified on five chromosomes 1 3 4 7 and 8 in the BC1 population by means of the Interval Mapping and restricted Multiple QTL Mapping methods. These QTLs came from S. pennellii with the exception of the minor QTL located on chromosome 8 which was provided by cv. Anl27. The main QTLs correspond to those detected on chromosomes 1 and 7. In the F2 population a QTL on chromosome 7 was identified on a similar region as that detected in the BC1 population. Marker segregation distortion was observed in this population in those areas where the QTLs of BC1 were detected. Furthermore we located two tomato .