tailieunhanh - báo cáo khoa học: "Global transcriptome analysis of two ameiotic1 alleles in maize anthers: defining steps in meiotic entry and progression through prophase I"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Global transcriptome analysis of two ameiotic1 alleles in maize anthers: defining steps in meiotic entry and progression through prophase I | Nan et al. BMC Plant Biology 2011 11 120 http 1471-2229 11 120 BMC Plant Biology RESEARCH ARTICLE Open Access Global transcriptome analysis of two ameioticl alleles in maize anthers defining steps in meiotic entry and progression through prophase I 1 2 1 2 1 Guo-Ling Nan Arnaud Ronceret Rachel C Wang John F Fernandes W Zacheus Cande and Virginia Walbot Abstract Background Developmental cues to start meiosis occur late in plants. Ameiotici Ami encodes a plant-specific nuclear protein AM1 required for meiotic entry and progression through early prophase I. Pollen mother cells PMCs remain mitotic in most ami mutants including ami-489 while aml-pral permits meiotic entry but PMCs arrest at the leptotene zygotene L Z transition defining the roles of AM1 protein in two distinct steps of meiosis. To gain more insights into the roles of AM1 in the transcriptional pre-meiotic and meiotic programs we report here an in depth analysis of gene expression alterations in carefully staged anthers at 1 mm meiotic entry and mm L Z caused by each of these ami alleles. Results mm and mm anthers of ami-489 and ami-pral were profiled in comparison to fertile siblings on Agilent 4 X 44 K microarrays. Both ami-489 and ami-pral anthers are cytologically normal at mm and show moderate transcriptome alterations. At the stage both mutants are aberrant cytologically and show more drastic transcriptome changes. There are substantially more absolute On Off and twice as many differentially expressed genes sterile versus fertile in ami-489 than in ami-pral. At mm a total of 4 418 genes are up- or down-regulated in either ami-489 or ami-pral anthers. These are predominantly stage-specific transcripts. Many putative meiosis-related genes were found among them including a small subset of allele-specific mis-regulated genes specific to the PMCs. Nearly 60 of transcriptome changes in the set of transcripts mis-regulated in both mutants N 530 are .