tailieunhanh - báo cáo khoa học: " Resistance to Plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Resistance to Plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses | Malacarne et al. BMC Plant Biology 2011 11 114 http 1471-2229 11 114 BMC Plant Biology RESEARCH ARTICLE Open Access Resistance to Plasmopara viticola in a grapevine segregating population is associated with stilbenoid accumulation and with specific host transcriptional responses 1 11 1 1 1 Giulia Malacarne Urska Vrhovsek Luca Zulini Alessandro Cestaro Marco Stefanini Fulvio Mattivi Massimo Delledonne2 Riccardo Velasco1 and Claudio Moser1 Abstract Background Downy mildew caused by the oomycete Plasmopara viticola is a serious disease in Vitis vinifera the most commonly cultivated grapevine species. Several wild Vitis species have instead been found to be resistant to this pathogen and have been used as a source to introgress resistance into a V. vinifera background. Stilbenoids represent the major phytoalexins in grapevine and their toxicity is closely related to the specific compound. The aim of this study was to assess the resistance response to P. viticola of the Merzling X Teroldego cross by profiling the stilbenoid content of the leaves of an entire population and the transcriptome of resistant and susceptible individuals following infection. Results A three-year analysis of the population s response to artificial inoculation showed that individuals were distributed in nine classes ranging from total resistance to total susceptibility. In addition quantitative metabolite profiling of stilbenoids in the population carried out using HPLC-DAD-MS identified three distinct groups differing according to the concentrations present and the complexity of their profiles. The high producers were characterized by the presence of trans-resveratrol trans-piceid trans-pterostilbene and up to thirteen different viniferins nine of them new in grapevine. Accumulation of these compounds is consistent with a resistant phenotype and suggests that they may contribute to the resistance response. A preliminary transcriptional study using cDNA-AFLP selected a set

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