tailieunhanh - Báo cáo hóa học: " Effect of amorphous silica nanoparticles on in vitro RANKL-induced osteoclast differentiation in murine macrophages"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effect of amorphous silica nanoparticles on in vitro RANKL-induced osteoclast differentiation in murine macrophages | Nabeshi et al. Nanoscale Research Letters 2011 6 464 http content 6 1 464 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Effect of amorphous silica nanoparticles on in vitro RANKL-induced osteoclast differentiation in murine macrophages Hiromi Nabeshi Tomoaki Yoshikawa Takanori Akase Tokuyuki Yoshida Saeko Tochigi Toshiro Hirai 12 12 12 12 12 2 Miyuki Uji Ko-ichi Ichihashi Takuya Yamashita Kazuma Higashisaka Yuki Morishita Kazuya Nagano 2 23 234 12 Yasuhiro Abe2 Haruhiko Kamada2 3 Shin-ichi Tsunoda2 3 4 Norio Itoh1 2 Yasuo Yoshioka2 3 and Yasuo Tsutsumi1 2 3 Abstract Amorphous silica nanoparticles nSP have been used as a polishing agent and or as a remineralization promoter for teeth in the oral care field. The present study investigates the effects of nSP on osteoclast differentiation and the relationship between particle size and these effects. Our results revealed that nSP exerted higher cytotoxicity in macrophage cells compared with submicron-sized silica particles. However tartrate-resistant acid phosphatase TRAP activity and the number of osteoclast cells TRAP-positive multinucleated cells were not changed by nSP treatment in the presence of receptor activator of nuclear factor kB ligand RANKL at doses that did not induce cytotoxicity by silica particles. These results indicated that nSP did not cause differentiation of osteoclasts. Collectively the results suggested that nanosilica exerts no effect on RANKL-induced osteoclast differentiation of cells although a detailed mechanistic examination of the nSP70-mediated cytotoxic effect is needed. Keywords silicon dioxide nanoparticle osteoclast differentiation Introduction Recently amorphous silica nanoparticles nSP with a controlled particle size below 100 nm have been a focus of investigations in various fields of industry. nSP have been used in a number of different industrial applications such as medicine .