tailieunhanh - Báo cáo hóa học: " A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA | Yu-Hong et al. Nanoscale Research Letters 2011 6 461 http content 6 1 461 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA Wang Yu-Hong1t Chen Rui2t and Li Ding 3 Abstract Background The recent advance in nanomaterial research field prompts the development of diagnostics of infectious diseases greatly. Many nanomaterials have been developed and applied to molecular diagnostics in labs. At present the diagnostic test of human papillomavirus HPV relies exclusively on molecular test. Hereon we report a rapid and facile quantum dots QDs and superparamagnetic nanoparticle-based hybridization assay for the detection of HPV 16 infections which combines the merits of superparamagnetic nanoparticles and QDs and wholly differs from a conventional hybridization assay at that the reaction occurs at homogeneous solution and total time for detection is no more than 1 h. Methods The probes were labeled with superparamagnetic nanoparticles and QDs. Sixty cervical swab samples were used to perform a hybridization assay with these probes and the results were compared with type-specific polymerase chain reaction PCR method. Results The statistic analysis suggests that there is no significant difference between these two methods. Furthermore this method is much quicker and easier than the type-specific PCR method. Conclusion This study has successfully validated the clinical performance of our hybridization assay. The advantages in the time of detection and ease of process endow this method with great potential in clinical usage especially mass epidemiological screening. Keywords HPV DNA quantum dots superparamagnetic nanoparticles hybridization cervical cancer Introduction Human papillomavirus HPV is a small non-enveloped DNA virus that merely infects human squamous epithelial cells. Its genome is a double-stranded circular DNA molecule of 8