tailieunhanh - báo cáo khoa học: " Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp.)"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp.) | Keeling et al. BMC Plant Biology 2011 11 43 http 1471-2229 11 43 BMC Plant Biology RESEARCH ARTICLE Open Access Transcriptome mining functional characterization and phylogeny of a large terpene synthase gene family in spruce Picea spp. 1 2 3 1 4 1 Christopher I Keeling Sabrina Weisshaar Steven G Ralph Sharon Jancsik Britta Hamberger Harpreet K Dullat Jorg Bohlmann 1 Abstract Background In conifers terpene synthases TPSs of the gymnosperm-specific TPS-d subfamily form a diverse array of mono- sesqui- and diterpenoid compounds which are components of the oleoresin secretions and volatile emissions. These compounds contribute to defence against herbivores and pathogens and perhaps also protect against abiotic stress. Results The availability of extensive transcriptome resources in the form of expressed sequence tags ESTs and full-length cDNAs in several spruce Picea species allowed us to estimate that a conifer genome contains at least 69 unique and transcriptionally active TPS genes. This number is comparable to the number of TPSs found in any of the sequenced and well-annotated angiosperm genomes. We functionally characterized a total of 21 spruce TPSs 12 from Sitka spruce P. sitchensis 5 from white spruce P. glauca and 4 from hybrid white spruce P. glauca X P. engelmannii which included 15 monoterpene synthases 4 sesquiterpene synthases and 2 diterpene synthases. Conclusions The functional diversity of these characterized TPSs parallels the diversity of terpenoids found in the oleoresin and volatile emissions of Sitka spruce and provides a context for understanding this chemical diversity at the molecular and mechanistic levels. The comparative characterization of Sitka spruce and Norway spruce diterpene synthases revealed the natural occurrence of TPS sequence variants between closely related spruce species confirming a previous prediction from site-directed mutagenesis and modelling. Background Conifer trees order Coniferales- Gymnosperms .

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